Construct SMR (pSMR-J18R), kindly provided by Zhang Q.(NCPGR,china), was mostly used for generation of rice mutant lines. Few lines were generated by transformation with constructs pCAMBIA1300 and pCAMBIA1301.

Left border of T-DNA (for SMR,pCAMBIA1300 and pCAMBIA1301): TGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGGGATCTG
GATTTTAGTACTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTA
TTTTACAAATACAAATACATACTAAGGGTTTCTTATATGCTCAACACATGAGCG
AAACCCTATAGGAACCCTAATTCCCTTATCTGGGAACTACTCACACATTATTAT
GGAGAAACTCGAGCTTGTCGATCGACAGATCCGGTCGGCATCTACTCTATTTCT
TTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGCGAGTACTTCTA
CACAGCCATCGGTCCAGACGGCCGCGCTTCTGCGGGCGATTTGTGTACGCCCGA
CAGTCCCGGCTCCGGATCGGACGATTGCGTCGCATCGACCCTGCGCCCAAGCTG
CATCATCGAAATTGCCGTCAACCAAGCTCTGATAGAGTTGGTCAAGACCAATGC
GGAGCATATACGCCCGGAGTCGTGGCGATCCTGCAAGCTCCGGATGCCTCCGCT
CGAAGTAGCGCGTCTGCTGCTCCATACAAGCCAACCACGGCCTCCAGAAGAAGA
TGTTGGCGACCTCGTATTGGGAATCCCCGAACATCGCCTCGCTCCAGTCAATGA
CCGCTGTTATGCGGCCATTGTCCGTCAGGACATTGTTGGAGCCGAAATCCGCGT
GCACGAGGTGCCGGACTTCGGGGCAGTCCTCGGCCCAAAGCATCAGCTCATCGA
GAGCC

Primer for TAIL-PCR
NTL1: 5' CACTCGTCCGAGGGCAAAGAAATAGA 3'
NTL2: 5' ATAGGGTTTCGCTCATGTGTTGAGC 3'
NTL3: 5' TTTCTAATTCCTAAAACCAAAATCCAGTAC 3'

Left border of T-DNA (for Ds): GTGAACGTCGGCTCGATTGCACCTGCGTTCAAATACTTTGCGATCGTGTTGCGC
GCCTGCCCGGTGCGTCGGCTGATCTCACGGATCGACTGCTTCTCTCGCAACGCC
ATCCGACGGATGATGTTTAAAAGTCCCATGTGGATCACTCCGTTGCCCCGTCGC
TCACCGTGTTGGGGGGAAGGTGCACATGGCTCAGTTCTCAATGGAAATTATCTG
CCTAACCGGCTCAGTTCTGCGTAGAAACCAACATGCAAGTTCCACCGGGTGCAA
AGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
AGTATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
TTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGGC

Primer for TAIL-PCR
T-DNA-1: 5'TCGAGGCGATCGTGAAGTTTCT 3'
T-DNA-2: 5' CGCCTATAAATACGACGGATCGTAA 3'
T-DNA-3: 5' TAATAACGCTGCGGACATCTA 3'