INTRODUCTION
 
 
 
 
 
 
 
  

Introduction


Using rice variety Zhonghua 11, a japonica rice, high efficiency agrobacterium- mediated transformation system was established and a population of rice mutant lines harboring T-DNA insertion has been generated. Currently(release March 17,2008, around 65,000 independent transgenic lines with T-DNA insertion have been obtained, in which 12,000 homozygous lines have been identified allowing the functional characterizations of recessive genes.

High-throughput thermal asymmetric interlaced (TAIL)-PCR was set up to efficiently recover the flanking sequence of the T-DNA left borders. Now more than 8,000 mutants are linked with the FST (flanking sequence tag) that can be located on the rice genome. All the FST are analyzed in the bioinformatics pipeline against release 5 of the TIGR Rice Pseudomolecules and Genome Annotation

Aside from sequence data, other information been integrated including: (1) seed quality measurement conducted on the 8,000 homozygous transformants, (2) phenotype observation during the growth of all the T1 and/or T2 progenies under agronomical conditions in the field.

Rice SHIP search is possible through either keywords, such as ID, gene No., gene name and mutant insertion site, or blast the database with nucleotide sequence or protein sequence. Alternatively, search can also be conducted by combining the different types of queries in an advanced search page.

We also welcome mutant and transgenic seeds donation from national and international researchers at any time.

 
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