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Sequence analysis
High-throughput thermal asymmetric interlaced (TAIL)-PCR (Liu Y. 1995) has been set up to efficiently recover the flanking sequence of the T-DNA left borders. Now more than 8,000 mutants are linked with the FST (flanking sequence tag) that can be located on the rice genome sequence. All the FST are analyzed in the bioinformatics pipeline against release 5 of the TIGR Rice Pseudomolecules and Genome Annotation.
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| FST distribution |
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